UW HomeGSTP HomeMy UWUW Search
UW Banner
Fostering new paradigms for the biological sciences

DOUGLAS PHANSTIEL

Chemistry Graduate Program

Department of Chemistry

Faculty Advisor: Joshua Coon

phanstiel@wisc.edu

890-0763

Resume 2007

        Doug Phanstiel

My GSTP research aims to use novel mass spectrometric instrumentation to characterize histone isoforms in differentiating human embryonic stem cells. Furthermore, we hope to correlate histone modification isoforms with changes in gene expression in hopes of better understanding how histone modifications regulate transcription and differentiation. Using a modified LTQ-Orbitrap mass spectrometer, we have analyzed and quantified histone H4 isoforms in human embryonic stem cells at multiple states of differentiation, and have identified over 50 previously unknown protein isoforms. I will be interrogating gene expression associated with regions on DNA where specific isoforms of histone H4 bind. I will be using ChIP-chip analysis to idenfity genes associated with dimethylated lysine 20. In addition, I will identify and quantify changes in histone H3 with respect to differentiation.

Search PubMed for Publications

Copyright © 2004 The Board of Regents of the University of Wisconsin System.
Site design Academic Web Pages